Solid-phase synthesis is currently the method of choice for synthesis of bio-molecules such as peptide, in which a stepwise strategy is used whereby reagents are added to the mixture and excess reagents are removed subsequently by simply washing the solid phase with a suitable solvent. The success of the solid-phase approach is closely tied to the performance of the solid support. Because of the poor swellability of Polystyrene (PS) resin and unstable nature of Polyethylene glycol (PEG) in acidic solution, there is a need to find a solid-phase support that is more homogeneous in terms of swellability and more stable in terms of compatibility with reaction conditions for solid phase synthesis.
Li-Resin has thus been developed and the resin has the following features:
(1) Good mechanical stability for practical loading, easy to handle versus hard to catch for PEG resin as fluffy flakes.
(2) Homogeneous swellability in most polar organic solvents.
(3) Robust chemical stability against strong acid and strong base.
(4) Good swellability in water or aqueous buffers, an advantageous feature as chemical manipulation on a bio-molecule can be feasible in aqueous system while the bio-molecule is still attached on the support, and additionally, biochemical applications can be developed such as on-resin screening of chemical libraries and affinity chromatography.
(5) The resin is being proven excellent for synthesis of bio-molecules with increasing number of peptides successfully synthesized in high yield and quality.
